[14] Analysis of hypersensitive sites in chromatin

摘要: Publisher Summary This chapter focuses on the analysis of hypersensitive sites in chromatin. Although precise cause and effect phenomenon nuclease hypersensitivity chromatin remains to be fully elucidated, identification cellular is useful as a marker nonnucleosomal organization that could also reflect an underlying sequence-specific protein-DNA interaction. The sequence experiments can serve general strategy for surveying eukaryotic gene or region special interest, with intention further analyzing those regions which break nucleosomal arrangement reflected by change sensitivity. Such vivo important study regulation it provides information interactions are actually occurring cell nucleus. concludes describing various properties restriction endonuclease protection limitations ExoIII techniques.