Mapping the nucleotide binding site of uncoupling protein 1 using atomic force microscopy.

摘要: A tight regulation of proton transport in the inner mitochondrial membrane is crucial for physiological processes such as ATP synthesis, heat production, or reactive oxygen species proposed uncoupling protein family members (UCP). Specific thus becoming increasingly important therapy obesity and inflammatory, neurodegenerative, ischemic diseases. We other research groups have shown previously that UCP1- UCP2-mediated inhibited by purine nucleotides. Several hypotheses been to explain inhibitory effect ATP, although structural details are still lacking. Moreover, unresolved mystery how UCP operates vivo despite permanent presence high (millimolar) concentrations mitochondria. Here we use topographic recognition (TREC) mode an atomic force microscope visualize UCP1 reconstituted into lipid bilayers analyze ATP–protein interaction at...