High speed optical nanoscopy by stimulated emission depletion (STED) with galvo mirrors

摘要: Stimulated emission depletion microscopy (STED) has been proved to be a feasible and straightforward method of breaking the conventional diffraction barrier in far-field. In this paper, we design setup home-built high speed STED microscope. Both excitation beam (488nm) (592nm) are provided by continuous wave lasers. By using pair galvo mirrors (TILL Yanus IV Digital Scan Head), realize pixel dwell time down 2 μs experiment which enables an acquisition frames per second imaging field 5*5 μm with individual size 10nm. The image process is controlled software Imspector. manuscript, give clear description on how build microscope also conduct several experiments evaluate its performance practice. A spatial resolution <100nm, well beyond demonstrated both nanoparticles biomedical samples. Featuring superresolution together speed, our big potential widely applied related scientific researches.