Identification and Quantification of Apo-lycopenals in Fruits, Vegetables, and Human Plasma

摘要: Research has suggested that lycopene may be metabolized by eccentric cleavage, catalyzed beta-carotene oxygenase 2, resulting in the generation of apo-lycopenals. Apo-6'-lycopenal and apo-8'-lycopenal have been reported previously raw tomato. We now show several other apo-lycopenals are also present processed foods, as well human plasma. Apo-lycopenal standards were prepared vitro oxidation lycopene, a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method using atmospheric pressure chemical ionization negative mode was developed to separate detect apo-6'-, apo-8'-, apo-10'-, apo-12'-, apo-14'-, apo-15'-lycopenal products formed reaction. Hexane/acetone extracts tomato, red grapefruit, watermelon, tomato analyzed, plasma individuals who had consumed juice for 8 weeks. Apo-6'-, apo-14'-lycopenals detected quantified all food tested, The sum 6.5 microg/100 g Roma 73.4 paste, 1.9 nmol/L conclude apo-lycopenals, addition apo-6'- -8'-lycopenal, lycopene-containing foods. In addition, presence derive from absorption directly and/or metabolism.