Ovarian morphological features and proteome reveal fecundity fitness disadvantages in β-cypermethrin-resistant strains of Blattella germanica (L.) (Blattodea: Blattellidae).

摘要: To evaluate whether the development of β-cypermethrin resistance in Blattella germanica (L.) (Blattaria: Blattellidae) affects fecundity fitness this insect and to determine underlying mechanism, we compared differences between β-cypermethrin-resistant (R) sensitive (S) strains B. germanica, observed physiological structural changes ovaries from an visual perspective, analyzed ovarian proteome using proteomic methods. The results showed that, with S strain R had a significantly higher ootheca shedding rate, lower number hatched surviving nymphs, female proportion population defective development. Ovarian analysis total 64 differentially expressed proteins strain, including 18 upregulated 46 downregulated proteins. Twenty-four were further studied, 14 successfully identified, which mainly classified into following categories: immunity-related proteins, development-related energy metabolism-related unknown functions. differential expression these reflects overall cell structure metabolism associated explains possible molecular mechanism disadvantages. In summary, can cause disadvantages germanica. metabolic deviations needed overcome adverse effects insecticides may result exchange that allocation and, ultimately, basic needs insect. cost due insecticide is critical delay evolution resistance.