N-Hydroxyguanidines oxidation by a N3S copper-complex mimicking the reactivity of Dopamine β-Hydroxylase

摘要: Abstract N -Aryl- ′-hydroxyguanidines are compounds that display interesting pharmacological properties but their chemical reactivity remains poorly investigated. Some of these substrates for the heme-containing enzymes nitric-oxide synthases (NOS) and act as reducing co-substrates copper-containing enzyme Dopamine β-Hydroxylase (DBH) [P. Slama, J.L. Boucher, M. Reglier, Biochem. Biophys. Res. Commun. 316 (2004) 1081–1087]. DBH catalyses hydroxylation important neurotransmitter dopamine into norepinephrine in presence both molecular oxygen a co-substrate. Although many molecules have been used DBH, interaction at active site role mechanism not clearly characterized. In present paper, we water-soluble copper-N 3 S complex mimics Cu B aromatic -hydroxyguanidines reducers to address this question. readily reduced copper(II) Cu(I) were oxidized nitrosoamidine previously observed reactions performed with purified DBH. These data describe first time -aryl- help understand copper