Keyhole limpet haemocyanin: negative staining in the presence of trehalose

摘要: Abstract Samples of unpurified and purified haemocyanin from the giant keyhole limpet Megathura crenulata have been studied by transmission electron microscopy (TEM) using mixtures trehalose with negative stains, uranyl acetate ammonium molybdate. Trehalose is a known protein preservative during air freeze drying, UV irradiation high temperatures, therefore offers possibility protecting proteins drying negatively-stained specimens their subsequent microscopical study. Evidence presented that possesses satisfactory stability within beam conventional room temperature, negative-staining studies. The combination stain generates deeper layer amorphous around (KLH) di- multidecamers, thereby increasing mobility generation range tilted images, alongside more usually observed end-on side-on image projections. concentration molybdate has increased to 4 5%, respectively, in presence 1% trehalose, order generate contrast. This because reduction net mass thickness dried stain-carbohydrate mixture compared for an equivalent alone. study these at low temperatures (−175°C) superior structural preservation.