Berberine ameliorates vascular dysfunction by a global modulation of lncRNA and mRNA expression profiles in hypertensive mouse aortae.
作者： Yan Zhang , Yan Zhang , Yi Zhang , Limei Liu , Limei Liu
DOI: 10.1371/JOURNAL.PONE.0247621
关键词:
摘要: Objective The current study investigated the mechanism underlying therapeutic effects of berberine in vasculature hypertension. Methods Angiotensin II (Ang II)-loaded osmotic pumps were implanted C57BL/6J mice with or without administration. Mouse aortae suspended myograph for force measurement. Microarray technology performed to analyze expression profiles lncRNAs and mRNAs aortae. These dysregulated expressions then validated by qRT-PCR. LncRNA-mRNA co-expression network was constructed reveal specific relationships. Results Ang Ⅱ resulted a significant increase blood pressure mice, which suppressed berberine. impaired endothelium-dependent aortic relaxation restored hypertensive mice. data revealed that 578 554 up-regulated, while 320 377 down-regulated Ⅱ; both reversed treatment. qRT-PCR validation results differentially expressed genes (14 6 mRNAs) completely consistent microarray data. GO analysis showed these verified significantly enriched terms "cellular process", "biological regulation" "regulation biological whilst KEGG identified vascular function-related pathways including cAMP signaling pathway, cGMP-PKG calcium pathway etc. Importantly, we observed lncRNA ENSMUST00000144849, ENSMUST00000155383, AK041185 majorly endothelial cells. Conclusion present suggest five NR_028422, AK041185, uc.335+ might serve critical regulatory roles targeting pivotal subsequently affecting pathways. Moreover, modulated berberine, therefore providing novel potential targets Furthermore, be involved preservation cell function.
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