Effect of anaesthetic technique on immune cell infiltration in breast cancer: a follow-up pilot analysis of a prospective, randomised, investigator-masked study.

摘要: Background Live animal studies using an inoculation model of breast cancer indicate that anaesthetic drugs and techniques differentially affect metastasis, inversely related to Natural Killer (NK) cell T lymphocyte levels. Clinical histological demonstrate the distribution these immune cells macrophages in intra-tumoral tissue can predict prognosis response therapy. No study has evaluated whether technique influences human infiltration. Materials methods Excised specimens from patients previously enrolled ongoing, prospective, randomised trial (NCT00418457) investigating effect on long-term outcome were immunohistochemically stained enable a colour deconvolution summate marked infiltration: CD56 (NK cells), CD4 (T helper CD8 suppressor cells) CD68 (macrophages). Patients receive either propofol-paravertebral with continuing analgesia (PPA, n=12) or balanced general anaesthesia opioid (GA, n=16) for 24 h postoperatively. Investigators masked group allocation. Results Normalised positive intensity values, (median (interquartile range (IQR)), lower GA121 (116-134) versus 136 (132-142), p=0.015. was also GA10.9 (5.5-27.8) PPA 19.7 (14.4-83.5), p=0.03 but 5.5 (4.0-9.75) 13.0 (5.0-14.5) respectively, p=0.24 CD 68 infiltration 5.8 (3.25-8.75) 8.0 (3.0-8.75), p=0.74 not significantly different. Conclusion induces increased levels NK into compared GA macro phages. This is consistent hypothesis may perioperative function conducive resisting recurrence metastasis.