Validation of the cytochemical section bioassay for thyroid stimulating antibodies.
作者： P. A. Ealey , P. P. A. Smyth
DOI: 10.1080/01971528008055783
关键词:
摘要: A cytochemical bioassay for thyroid stimulators which utilised 12 micrometer sections of guinea pig as the target tissue was validated and applied to measurement stimulating antibodies (T.S.Ab.) in human plasma. T.S.H. T.S.Ab. could be distinguished assay by times at they produced maximal responses; 90 seconds 180 Homogeneity responses stimulation relatively uniform throughout enabling simultaneous several plasma samples per pig. Sensitivity 1.5 x 10(-9) microU/l MRC L.A.T.S.-B. Antiserum H-T.S.H. no significant change T.S.Ab.-like activity when added from a thyrotoxic patient. Both anti-IgG aliquots homogenate patient diminished concentration dependent manner. present titres 1/100 1/100,00 13 hyperthyroid patients tested date.
sci-hub.se 参考文章(20)
J.M. MCKENZIE, M. ZAKARIJA, LATS in Graves' disease. Recent Progress in Hormone Research. ,vol. 33, pp. 29- 57 ,(1977) , 10.1016/B978-0-12-571133-3.50009-X
LUCILLE BITENSKY, J. ALAGHBAND-ZADEH, J. CHAYEN, STUDIES ON THYROID STIMULATING HORMONE AND THE LONG‐ACTING THYROID STIMULATING HORMONE Clinical Endocrinology. ,vol. 3, pp. 363- 374 ,(1974) , 10.1111/J.1365-2265.1974.TB01811.X
JACQUES ORGIAZZI, DONALD E. WILLIAMS, INDER J. CHOPRA, DAVID H. SOLOMON, Human Thyroid Adenyl Cyclase-Stimulating Activity in Immunoglobulin G of Patients with Graves' Disease The Journal of Clinical Endocrinology and Metabolism. ,vol. 42, pp. 341- 354 ,(1976) , 10.1210/JCEM-42-2-341
RosalindS. Brown, StephenL. Pohl, IvorM.D. Jackson, Seymour Reichlin, DO THYROID-STIMULATING IMMUNOGLOBULINS CAUSE NON-TOXIC AND TOXIC MULTINODULAR GOITRE? The Lancet. ,vol. 311, pp. 904- 906 ,(1978) , 10.1016/S0140-6736(78)90682-7
E. K. MASON, B. S. HETZEL, B. F. GOOD, N. S. STENHOUSE, An Improved Bio-assay for the Determination of Long-Acting Thyroid Stimulator (LATS) The Journal of Clinical Endocrinology and Metabolism. ,vol. 27, pp. 1529- 1539 ,(1967) , 10.1210/JCEM-27-11-1529
B SMITH, THYROID-STIMULATING IMMUNOGLOBULINS IN GRAVES' DISEASE The Lancet. ,vol. 304, pp. 427- 430 ,(1974) , 10.1016/S0140-6736(74)91815-7
O TROWELL, The culture of mature organs in a synthetic medium Experimental Cell Research. ,vol. 16, pp. 118- 147 ,(1959) , 10.1016/0014-4827(59)90201-0
TOSHIMASA ONAYA, MASANOBU KOTANI, TAKASHI YAMADA, YUKIO OCHI, New in vitro tests to detect the thyroid stimulator in sera from hyperthyroid patients by measuring colloid droplet formation and cyclic AMP in human thyroid slices. The Journal of Clinical Endocrinology and Metabolism. ,vol. 36, pp. 859- 866 ,(1973) , 10.1210/JCEM-36-5-859
JOHN O’DONNELL, KRINOS TROKOUDES, JAY SILVERBERG, VAS ROW, ROBERT VOLPÉ, Thyrotropin displacement activity of serum immunoglobulins from patients with Graves' disease. The Journal of Clinical Endocrinology and Metabolism. ,vol. 46, pp. 770- 777 ,(1978) , 10.1210/JCEM-46-5-770
YOSHIMASA SHISHIBA, YOSHIE MIYACHI, MASARU TAKAISHI, YASUNORI OZAWA, LATS-Protector Activity in Thyrotoxicosis Measured by Thyroidal Intracellular Colloid Droplet Formation* The Journal of Clinical Endocrinology and Metabolism. ,vol. 46, pp. 841- 848 ,(1978) , 10.1210/JCEM-46-5-841