ATPase inhibition and electrophysiological change caused by DDT and related neuroactive agents in lobster nerve

摘要: Abstract In an attempt to elucidate the biochemical and physiological mechanisms of action DDT on nerve membrane, experiments have been performed study effects related chemicals ATPases electrical activity lobster nerves. were obtained from nerves walking legs lobster, Homarus americanus . The optimum conditions for maximum ATPase were: Na + 320 mM, K 20 Mg 2+ 1 mM at pH 7.6–8.0. rigid requirements , suggest that major portion in preparations consists -K -Mg ATPases. inhibited a It was found, however, only DDT-sensitive could be by high concentration ouabain. view possibility are not ones involved active transport systems, variety neuroactive agents which known induce conductance changes examined their actions preparations. majority cause electrophysiological responses similar found potent inhibitors. A correlation between degree inhibition symptoms poisoning.