Synthesis of luminescent biotinylated multivalent dendrimer encapsulated quantum dots and investigation on its physico-chemical interactions with biological receptor avidin

摘要: Abstract It is challenging to design and characterize molecular recognition pair consisting of both targeting imaging property it also particular interest inquire into the limits affinity that can be achieved with designed bio-conjugate. This paper presents functionalization luminescent quantum dots (QDs) employing multivalent dendrimer molecules conjugated biotin as moiety photo-physical thermodynamic behavior this bio-conjugate in terms highly affinic binding avidin. demonstrated fluorescence intensity QDs remained almost unaffected when synthesized conjugate interacts avidin considered an important requisite for applications sensing. The observed changes average emission lifetime well values components indicate tryptophan Calorimetric investigation reveals biotin-dendrimer exothermic process accompanied favorable enthalpy (ΔH = −21.6 kcal/mol) unfavorable entropy (ΔS = −43.7 cal/mol/deg). These findings may help multiplexed assays where based probe are immobilized via avidin-biotin interactions which have implications development immuno-histochemical techniques.