Phencyclidine blocks two potassium currents in spinal neurons in cell culture

摘要: Abstract We studied the effects of phencyclidine (PCP) on transient and delayed outward K− currents recorded from spinal cord neurons grown (10–20 days) in cell culture. Sodium channels were blocked with tetrodotoxin (1 μM) solutions containing low calcium concentrations presence Mg2− or Co2− (5 mM) used to reduce Ca2− currents. PCP decreased amplitude prolonged decay phase action potentials at a holding potential −70 mV. (0.1–0.5 was more effective than tetraethylammonium (TEA) 4-aminopyridine (4-AP) reducing both The current during control experiments always larger that current. It appeared 4-AP potent blocking current, while TEA (10 modified effectively. Both also reduced by about 10% when soma perfused Co2−. This suggested small fraction total is activated PCP-induced blockade central coupled profound synaptic drug may provide basis for explaining psychopathology this hallucinogenic agent.