Membrane potential defect in hygromycin B-resistant pma1 mutants of Saccharomyces cerevisiae.
作者： D S Perlin , C L Brown , J E Haber
DOI: 10.1016/S0021-9258(19)81331-5
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摘要: The proton transport properties of hygromycin B-resistant pma1 mutants which show kinetic defects in the plasma membrane H+-ATPase were examined. It was found that net efflux, as measured by whole cell medium acidification presence 25 mM KCl, similar for normal and mutant cells. However, absence added extent efflux considerably less wild type than cellular potential implicated an important factor regulating determined from [14C]tetraphenylphosphonium uptake studies to be depolarized mutants. growth cells, is normally inhibited B at 200 micrograms/ml, resistant antibiotic addition 50 KCl medium. These results suggest electrogenic behavior may altered resistance mediated via depolarization potential.
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