Evaluating the reliability of microsatellite genotyping from low-quality DNA templates with a polynomial distribution model

摘要: Molecular studies using trace DNA, such as from museum specimens, ancient or forensic samples and obtained noninvasively, often have a common problem of low quality DNA templates. Amplification errors, allelic dropout false allele, may arise during polymerase chain reaction (PCR) samples. A mathematical model which treats homozygotes heterozygotes discriminately has been developed to measure sample compute the confidence level multiple-tube approaches. We use plucked hair collected 26 individual Sichuan snub-nosed monkeys (Rhinopithecus roxellana) test model. In this case, 99% can be achieved by three positive PCRs. If is very poor requires many PCR replicates, an alternative multiple-step genotyping method recommended. This enables researchers optimize experimental protocols through pilot obtain reliable genetic information noninvasive sampling method.