Peroxidase from Strawberry Fruit (Fragaria ananassa Duch.): Partial Purification and Determination of Some Properties

摘要: In this work, peroxidase from strawberry fruit was detected, partially purified, and characterized. The total enzyme extract (both soluble associated to membranes) purified by means of (NH 4 ) 2 SO precipitation, molecular exclusion chromatography, cationic exchange chromatography. purification grade achieved near 35. Effects temperature pH, stability against thermal were analyzed on both crude extracts. maximum activity observed at 30 °C pH 6.0. showed low maintained activities equal or greater than 50% its value in the 4-11 range. Two isoenzymes detected ; they basic type (isoelectric points 9.5-10.0) had masses 58.1 65.5 kDa. Strawberry decreased remarkably as ripened found primarily a membrane-bound form. Maximum specific small green large ripening stages.