Innovative metal oxide-based substrates for DNA microarrays

摘要: Abstract In the present report, we propose a novel approach to synthesize DNA microarrays that employs immobilization of nucleic acid molecules onto zinc and iron oxide surfaces through their phosphate backbone. Oxide films were prepared by sol–gel technique resulting characterized especially with respect surface chemistry morphological features both X-ray photoelectron spectroscopy (XPS) atomic force microscopy (AFM). ZnO annealed at T ⩽ 300 °C show most promising be employed for microarray preparation, i.e. high density binding sites (hydroxyl groups), smooth homogeneous surfaces, optical transmittance in visible spectral range suitable detection using fluorescence, easy handling during preparation procedures. The analysis retention on layers was performed scanning dye-labelled previously printed substrate robotic arm. Clearly spots regular shape revealed above background noise indicating anchoring treated is efficient does not interfere hybridization processes. use suitably engineered film makes strategy ideal facile, efficient, cost-effective manufacturing microarrays.