Blood-based liquid biopsy for comprehensive cancer genomic profiling using next-generation sequencing: an emerging paradigm for noninvasive cancer detection and management in dogs

摘要: This proof-of-concept study demonstrates that blood-based liquid biopsy using next generation sequencing of cell-free DNA can noninvasively detect multiple classes genomic alterations in dogs with cancer, including originate from spatially separated tumor sites. Eleven a variety confirmed cancer diagnoses (including localized and disseminated disease) who were scheduled for surgical resection, 5 presumably cancer-free dogs, enrolled. Blood was collected each subject, tissue samples during surgery 9 the subjects. All analyzed an advanced prototype novel test designed to interrogate detection, characterization, management dogs. In patients matched plasma samples, pre-surgical testing identified alterations, single nucleotide variants copy number variants, independently detected corresponding samples. Importantly, observed same demonstrating potential comprehensive profiling heterogeneous tumors. Among 3 post-surgical blood remained detectable one patient incomplete suggesting utility noninvasive detection minimal residual disease following curative-intent treatment. Liquid allows cancer-associated simple draw has overcome limitations tissue-based posed by tissue-level heterogeneity.