Tissue and subcellular distribution of mercaptopyruvate sulfurtransferase in the rat: confocal laser fluorescence and immunoelectron microscopic studies combined with biochemical analysis.

摘要: In our previous study, we found that mercaptopyruvate sulfurtransferase (MST) was evolutionarily related to mitochondrial rhodanese. To elucidate the difference between MST and rhodanese, tissue, cellular, subcellular distribution of rat determined biochemically immunohistochemically by using anti-MST antibody raised in rabbit. an immunohistochemical tetramethyl rhodamine isothiocyanate-conjugated phalloidin against F-actin fluorescein goat anti-rabbit immunoglobulin as a secondary were used for double fluorescent staining. They detected confocal laser fluorescence microscopy. immunoelectron microscopic study hepatocyte renal tubular epithelium, postembedding immunogold method used. Biochemical studies including western blot analyses various tissues fractions liver also performed. widely distributed but cellular be different each tissue. predominantly localized proximal epithelium kidney, pericentral hepatocytes liver, cardiac cells heart, neuroglial brain. This immunocytochemical both mitochondria cytoplasm.