Gene expression profiles in rat lung after inhalation exposure to C60 fullerene particles.

摘要: Abstract Concern over the influence of nanoparticles on human health has risen due to advances in development nanotechnology. We are interested pulmonary system at a molecular level. In this study, gene expression profiling rat lung after whole-body inhalation exposure C60 fullerene (0.12 mg/m3; 4.1 × 104 particles/cm3, 96 nm diameter) and ultrafine nickel oxide (Uf-NiO) particles (0.2 mg/m3; 9.2 × 104 59 nm as positive control were employed gain insights into these events. response for 6 h day, 4 weeks (5 days week), located alveolar epithelial cells 3 post-exposure engulfed by macrophages both 1 month post-exposures. Gene profiles revealed that few genes involved inflammatory response, oxidative stress, apoptosis, metalloendopeptidase activity up-regulated post-exposure. Only some associated with immune process, including major histocompatibility complex (MHC)-mediated immunity up-regulated. These results significantly different from those Uf-NiO which induced high chemokines, matrix metalloproteinase 12 (Mmp12), suggesting lead acute inflammation period, damaged tissues repaired period. suggest might not have severe toxicity under condition.