Prevalence of porcine reproductive and respiratory syndrome virus detection in aborted fetuses, mummified fetuses and stillborn piglets using quantitative polymerase chain reaction.
作者： Em-on OLANRATMANEE , Piya WONGYANIN , Roongroje THANAWONGNUWECH , Padet TUMMARUK
DOI: 10.1292/JVMS.14-0480
关键词:
摘要: The objective of the present study was to investigate prevalence porcine reproductive and respiratory syndrome (PRRS) virus detection in aborted fetuses (n=32), mummified (n=30) stillborn piglets (n=27) from 10 swine herds Thailand using quantitative polymerase chain reaction (qPCR). Pooled organs umbilical cord each fetus/piglet were homogenized subjected RNA extraction cDNA synthesis. qPCR carried out on ORF7 PRRS viral genome fluorogenic probes for amplified product detection. results revealed that 67.4% (60/89) specimens contained virus. found 65.6% (21/32) fetuses, 63.3% (19/30) 74.1% (20/27) (P=0.664). Genotype 1, genotype 2 mixed genotypes detected 19.1% (17/89), 25.8% (23/89) 22.5% (20/89) specimens, respectively (P=0.316). antigen retrieved both non-PRRS-vaccinated (68.2%, 45/66) PRRS-vaccinated (65.2%, 15/23) (P=0.794). These findings indicated these are important sources load shedding within herd. Thus, intensive care routine management dead virus-positive should be emphasized.
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