Injectable chitosan/gelatin/bioactive glass nanocomposite hydrogels for potential bone regeneration: In vitro and in vivo analyses
作者： Cheisy D.F. Moreira , Sandhra M. Carvalho , Rodrigo M. Florentino , Andressa França , Bárbara S. Okano
DOI: 10.1016/J.IJBIOMAC.2019.03.237
关键词:
摘要: The present work describes in vitro and vivo behaviors of thermosensitive composite hydrogels based on polymers/bioactive glass nanoparticles. Assays SBF (simulated body fluid) solution showed that loss hydrogel mass was decreased by 4.3% when bioactive nanoparticles (nBG) were incorporated, confirmed the bioactivity nBG containing hydrogels. In assays demonstrated cytocompatibility with encapsulated rat bone marrow mesenchymal stem cells (BMSC). Crystal violet a 27% increase cell viability these seeded nBG. biocompatibility examined injecting into dorsum Swiss rats. results indicated prepared nontoxic upon subcutaneous injection, could be candidates for safe situ gel-forming system. Injection tibial defect allowed preliminary evaluation hydrogels' regenerative potential. Micro Computed Tomography analysis suggested more new tissue formed defects treated Taken together, our data suggest developed injectable possess properties which make them suitable use as temporary matrices regeneration.
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