Production of protocatechuic acid by Corynebacterium glutamicum expressing chorismate-pyruvate lyase from Escherichia coli.

摘要: Protocatechuic acid (3,4-dihydroxybenzoic acid; PCA) serves as a building block for polymers and pharmaceuticals. In this study, the biosynthetic pathway PCA from glucose was engineered in Corynebacterium glutamicum. The to PCA-employed elements of chorismate by using chorismate-pyruvate lyase (CPL) 4-hydroxybenzoate hydroxylase (4-HBA hydroxylase). As C. glutamicum has potential synthesize aromatic amino intermediate possesses 4-HBA hydroxylase, we focused on expressing Escherichia coli CPL phenylalanine-producing strain ATCC21420. To secrete PCA, gene (ubiC) encoding E. expressed ATCC 21420 (strain F(UbiC)). formation 28.8 mg/L extracellular (36 h) 213 ± 29 mg/L (80 h) obtained F(UbiC) glucose. ATCC21420 also found produce PCA. fermentation performed bioreactor at optimized pH 7.5. produced 615 ± 2.1 mg/L 50 g/L after 72 h. Further, fed-batch with feedings every 24 h. maximum production (1140.0 ± 11.6 mg/L) achieved when 117.0 g/L added over 96 h fermentation.