Structure of an E6AP-UbcH7 Complex: Insights into Ubiquitination by the E2-E3 Enzyme Cascade

摘要: The E6AP ubiquitin-protein ligase (E3) mediates the human papillomavirus-induced degradation of p53 tumor suppressor in cervical cancer and is mutated Angelman syndrome, a neurological disorder. crystal structure catalytic hect domain reveals bilobal with broad cleft at junction two lobes. consists conserved residues whose mutation interferes ubiquitin-thioester bond formation site syndrome mutations. bound to UbcH7 ubiquitin-conjugating enzyme (E2) determinants E2-E3 specificity provides insights into transfer ubiquitin from E2 E3.