Characterization of Physical Gap Sizes at Human Telomeres

摘要: Genome-wide physical and genetic mapping efforts have not yet fully addressed the problem of closure at telomeric ends human chromosomes. Targeted cloning mouse telomeres succeeded in identifying unique sequences most telomeres, but gap sizes between these telomere clones distal markers on integrated genetic/physical maps remain largely unknown. As regions are known to be gene-rich genome, filling gaps should a high priority completion Human Genome Project. We reported previously first generation set sequence probes for regions. Of 41 regions, 33 were represented by with distance (≤300 kb) from end chromosome; remaining eight had been identified map. four 9p, 12p, 15q, 16p. To determine size chromosomes five other interphase FISH analysis was performed measure each clone corresponding marker. These studies provide estimates ranging 1 Mb, thus defining task gaps.