作者: Yenilda Laguna-Ibarra ,
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摘要: Se realizo la propagacion in vitro de Senecio calvus como una alternativa para su conservacion . Las yemas se desinfectaron con HgCl 2 (0.01 y 0.1%) sin adicion periodica de antibioticos. El medio cultivo Murashige & Skoog (1/2) mas sacarosa 2%, fitagel 0.3% pH 5.7 fue suplementado bencilaminopurina multiplicacion; mientras que el enraizamiento uso acido naftalenacetico o giberelico. agregaron tratamientos regulador crecimiento. Para aclimatacion evaluaron dos sustratos naturales. mayor porcentaje desinfeccion obtuvo 0.1% por 5 minutos aplicacion rifampicina (72 µg·mL -1 ) nistatina (200 cada tres dias 18 dias. La incremento sobrevivencia las introducidas plantas brotes; pero no encontro diferencias significativas en numero brotes planta. giberelico tuvo efecto positivo sobre indujo formacion callos. mejor sustrato tierra + musgo (1:1). ABSTRACT In propagation of calvus was carried out as an alternative for its conservation. The buds were disinfected with and without periodic addition of antibiotics. culture medium plus 2% sucrose phytagel 0.3%, was supplemented benzylaminopurine multiplication; while the rooting naphthaleneacetic acid or gibberellic used. Treatments growth regulator added. For acclimation two natural substrates evaluated. highest percentage disinfection obtained 0.1% for minutes adding rifampicin μg·mL-1) nystatin every three days per days. Benzylaminopurine increased survival introduced shooted plants; however number shoots plant did not show significant differences. Gibberellic acidhad a positive effect on induced callusing formation. best substrate acclimatization soil moss (1: 1). Key words : gibberellic acid, Asteraceae, benzylaminopurine, culture.