[3] Assaying binding of nerve growth factor to cell surface receptors

摘要: Publisher Summary This chapter describes methods both for the iodination of nerve growth factor (NGF) and assaying NGF receptors by reversible binding techniques mentions alternative employed other investigators. The explains that plays an essential role in development sympathetic sensory nervous systems, terms promoting neuronal survival possibly chemotactically directing axons toward appropriate target tissues. It can be prepared milligram quantities from submaxillary glands adult male mice a variety previously reported methods. reviews important breakthrough attempting to understand mechanism action NGF, which has been identification specific cell surface this hormone. Radioiodinated derivatives retained biological activity, were able show [ 125 I] cells respond