作者: Sean Hoban , Gayle Volk , Kanin J. Routson , Christina Walters , Chris Richards
DOI: 10.1007/978-3-319-95101-0_8
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摘要: Sampling seed from natural populations of crop wild relatives requires choice the locations and amount to sample. While this may seem like a simple choice, in fact careful planning collector’s sampling strategy is needed ensure that collection will contain high genetic variation, which turn for potential breeding or selection. Here we first describe different conservation targets intensities at collector might We then review research on appropriate number populations, plants, seeds collect, methodologies available helping make these decisions. suggest samplers reconsider long-standing minimum 50 samples per population, as be insufficient some cases. explain optimal samples, can determined with modeling approaches (niche modeling, survey, simulations) use knowledge species’ inherent traits (e.g., outcrossing rates) geographic distribution. Lastly, practical aspects sampling, including need return source population collect due change over time additional (sometimes 10 times much seed) account loss during storage use. also emphasize utility collecting abundant spatial environmental data well considering how used accompanying analyses, order ex situ collections useful many years come.