MiR-133a in Human Circulating Monocytes: A Potential Biomarker Associated with Postmenopausal Osteoporosis

作者: Xian-Hao Peng , Xiang Fang , Joan M. Lappe , Robert R. Recker , Peng Xiao

DOI: 10.1371/JOURNAL.PONE.0034641

关键词:

摘要: Background Osteoporosis mainly occurs in postmenopausal women, which is characterized by low bone mineral density (BMD) due to unbalanced resorption osteoclasts and formation osteoblasts. Circulating monocytes play important roles osteoclastogenesis acting as osteoclast precursors secreting osteoclastogenic factors, such IL-1, IL-6 TNF-α. MicroRNAs (miRNAs) have been implicated biomarkers various diseases. The present study aimed find significant miRNA human circulating underlying osteoporosis. Methodology/Principal Findings We used ABI TaqMan® array followed qRT-PCR validation identify 10 high BMD Caucasian women. MiR-133a was upregulated (P=0.007) the compared with groups analyses, also validated (P=0.044). We performed bioinformatic target gene analysis found three potential osteoclast-related genes, CXCL11, CXCR3 SLC39A1. In addition, we Pearson correlation analyses between expression levels of miR-133a genes 20 did negative correlations all though not significant. Conclusions/Significance This first vivo novel osteoporosis. Our results suggest that a biomarker for

参考文章(62)
T. Eskildsen, H. Taipaleenmaki, J. Stenvang, B. M. Abdallah, N. Ditzel, A. Y. Nossent, M. Bak, S. Kauppinen, M. Kassem, MicroRNA-138 regulates osteogenic differentiation of human stromal (mesenchymal) stem cells in vivo Proceedings of the National Academy of Sciences of the United States of America. ,vol. 108, pp. 6139- 6144 ,(2011) , 10.1073/PNAS.1016758108
Jian-Fu Chen, Elizabeth M Mandel, J Michael Thomson, Qiulian Wu, Thomas E Callis, Scott M Hammond, Frank L Conlon, Da-Zhi Wang, None, The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation Nature Genetics. ,vol. 38, pp. 228- 233 ,(2006) , 10.1038/NG1725
Y Fujikawa, J M Quinn, A Sabokbar, J O McGee, N A Athanasou, The human osteoclast precursor circulates in the monocyte fraction. Endocrinology. ,vol. 137, pp. 4058- 4060 ,(1996) , 10.1210/ENDO.137.9.8756585
Tomoyuki Nakasa, Hayatoshi Shibuya, Yoshihiko Nagata, Takuya Niimoto, Mitsuo Ochi, The inhibitory effect of microRNA-146a expression on bone destruction in collagen-induced arthritis Arthritis & Rheumatism. ,vol. 63, pp. 1582- 1590 ,(2011) , 10.1002/ART.30321
M. E. Cohen-Solal, F. Boitte, O. Bernard-Poenaru, M. A. Denne, A. M. Graulet, M. Brazier, M. C. de Vernejoul, Increased Bone Resorbing Activity of Peripheral Monocyte Culture Supernatants in Elderly Women The Journal of Clinical Endocrinology and Metabolism. ,vol. 83, pp. 1687- 1690 ,(1998) , 10.1210/JCEM.83.5.4808
Qiang Zhou, Zhi-Ning Zhao, Jing-Tao Cheng, Bin Zhang, Jie Xu, Fei Huang, Rui-Ni Zhao, Yong-Jin Chen, Ibandronate promotes osteogenic differentiation of periodontal ligament stem cells by regulating the expression of microRNAs. Biochemical and Biophysical Research Communications. ,vol. 404, pp. 127- 132 ,(2011) , 10.1016/J.BBRC.2010.11.079
Nikolaus Rajewsky, microRNA target predictions in animals. Nature Genetics. ,vol. 38, ,(2006) , 10.1038/NG1798
Yeon Jeong Kim, Sang Woo Bae, Sung Sook Yu, Yong Chan Bae, Jin Sup Jung, miR-196a regulates proliferation and osteogenic differentiation in mesenchymal stem cells derived from human adipose tissue. Journal of Bone and Mineral Research. ,vol. 24, pp. 816- 825 ,(2009) , 10.1359/JBMR.081230
G. Atzmon, X. M. Yang, R. Muzumdar, X. H. Ma, I. Gabriely, N. Barzilai, Differential gene expression between visceral and subcutaneous fat depots. Hormone and Metabolic Research. ,vol. 34, pp. 622- 628 ,(2002) , 10.1055/S-2002-38250