Neuroprotection by NGF in the PC12 In Vitro OGD Model

摘要: : Neurodegenerative disorders and chronic disability due to stroke in the brain or spinal cord afflict a large sector of population. To investigate mechanism involved ischemic develop neuroprotective drugs/therapies, vivo vitro, pharmacological models are needed. cellular molecular mechanisms nerve growth factor (NGF), member nervous system neurotrophin family factors, under ischemia, we used an oxygen-glucose-deprivation (OGD) device pheochromocytoma PC12 cells exposed paradigm insult. Pretreatment cultures with 50 ng/mL NGF, 18 h prior OGD insult, conferred 30% neuroprotection. Time-course experiments showed marked activation ERK, JNK, p-38 MAPK isoforms during phase, but not reperfusion. resulted 50% attenuation OGD-induced JNK 1, 20% α β, respectively. The effect NGF on gene expression model using Affymatrix Rat DNA-Microarray technology indicates that only 6% genes differentially regulated (induced/suppressed) by insult and/or NGF. These findings support notion pretreatment confers neuroprotection from phenomenon coincidentally related differential inhibition stress kinase expression. This may be useful neurotoxicity NGF-induced neuroprotection, generate novel therapeutic concepts for treatment.