Cytochrome P450 monooxygenase specific activity reduction in wheat Triticum aestivum induced by soil roxithromycin stress

摘要: Roxithromycin, as widely used medicine and livestock growth promoter, arouses concern because its occurrence persistence in soil environments. However, effects of roxithromycin higher plants are still vague. Accordingly, we hypothesized that roxithromycin-contaminated may exhibits ecotoxicological wheat (Triticum aestivum). In this study, induced by a gradient concentration stress (0.01, 0.1, 1, 10, 100 mg·kg–1) was investigated 7-d test T. aestivum. Results indicated the specific activity cytochrome P450 (CYP450) monooxygenase decreased dramatically with soil. The IC50 value 8.78 mg·kg–1 roxithromycin. On contrary, related endpoints (i.e., germination percentage, biomass height), content soluble protein CYP450 content), superoxide dismutase (SOD) failed to reveal roxithromycin-induced effects. Further analysis revealed reduction enzymatic mechanism mediated, other than oxidative induced. We conclude declined aestivum inhibition mechanism. efforts can include, but not limited to, investigation joint combined exposure pesticides evaluation similar plants.