Simultaneous determination of organic acids and vitamin C in green beans by liquid chromatography

摘要: A method is described for determining and quantitating organic acids (oxalic, malic, citric, fumaric) vitamin C by liquid chromatography with a UV-visible detector that allows simultaneous monitoring at 2 wavelengths. The was applied to samples of green beans (Phaseolus vulgaris L.). Recoveries were 97.8% oxalic acid, 98.9% malic 98.7% citric 99.2% fumaric 98.5% C. Method precisions (coefficients variation) 1.7% 0.8% 0.9% 1.5% 1.2% Measurement 1.32% 0.33% 0.62% 1.01% 0.39% Limits detection 0.025 mg/mL 0.022 0.024 1.0 x 10(-4) 2.7