Kinetic studies on the electron transfer between various c-type cytochromes and iron (III) using a voltammetric approach

摘要: Abstract Cyclic voltammetry was used here to investigate the kinetics of electron transfer between a soluble iron(III) complex and several cytochromes isolated from Desulfovibrio Desulfuromonas species. The second order rate constants were calculated found be in 10 5 –10 6  M −1  s range. Their values are discussed point view redox potentials, heme content pI value proteins. ability poly(ester–sulfonic acid) ionomer (Eastman AQ-29D) entrap positively charged made it possible study immobilized When incorporated into this polymer matrix, diffusion limitations lead 50 times lower catalytic efficiency as compared catalysis with protein solution. results demonstrate that only bishistidinyl iron coordination act metal reducers whereas mitochondrial c-type do not. This approach opens new pathways for use sulfur sulfate reducing bacteria bioremediation contaminated waters waste streams. Processes involving entrapped enzymes reactors could developed according activity their polyhemic cytochromes.