A simplified preservation method for lungs donated after cardiac death.

摘要: Background The shortage of donor lungs restricts the number lung transplantations that can be performed. However, extension pool using organs donated after cardiac death (DCD) could potentially increase patients who undergo transplantation. To establish acceptance among hospital personnel and donor's next kin for uncontrolled DCD procedure we proposed a simplified preservation regime intrapleural cooling lungs. Methods In an model, 12 pigs were randomized to either standard method with two bilateral chest tubes intermittent pleural fluid exchanges, or simplified, less-invasive single tube filling space without exchange. Lungs explanted graft function was assessed during ex vivo perfusion (EVLP) by histologic examination. Results Although mean temperature 120 minutes significantly higher in cooled (25.9°C vs 13.5°C), this did not affect oxygenation capacity, pulmonary vascular resistance dynamic compliance lungs, as recorded EVLP. Furthermore, no differences found between preserved methods respect wet/dry ratio, levels myeloperoxidase bronchoalveolar lavage, at Conclusions technique results but does EVLP, which implies less invasive used setting. This is another step forward development routine DCD.