Fluorescence Spectroscopy: Applications in Chemical Biology

摘要: Fluorescence spectroscopy in all of its variations can be considered among the most powerful types analysis available to chemical biology. However, useful almost applications are dependent on optimal labeling biomolecules with a fluorophore and appropriate choice analytical technique. In this article, we examine contributions fluorescent biology three inter-related sections. We first properties common fluorophores from many disparate structural functional classes, which includes discussion their individual benefits liabilities context application. The conjugation chemistries used attach myriad next reviewed. As each class biomolecule differs both structure function, focus here is strategies for specific different groups. Last, some major associated biologic questions that addressed them covered briefly.