A systematic study of the whole genome sequence of Amycolatopsis methanolica strain 239T provides an insight into its physiological and taxonomic properties which correlate with its position in the genus.

摘要: The complete genome of methanol-utilizing Amycolatopsis methanolica strain 239T was generated, revealing a single 7,237,391 nucleotide circular chromosome with 7074 annotated protein-coding sequences (CDSs). Comparative analyses against the japonica MG417-CF17T, mediterranei U32 and orientalis HCCB10007 revealed broad spectrum genomic structures, including various sizes, core/quasi-core/non-core configurations different kinds episomes. Although polyketide synthase gene clusters were absent from A. methanolica genome, 12 related to biosynthesis other specialized (secondary) metabolites identified. Complete pathways attributable facultative methylotrophic physiology 239T, both mdo/mscR encoded methanol oxidation hps/hpi formaldehyde assimilation via ribulose monophosphate cycle, identified together evidence that latter might be result horizontal transfer. Phylogenetic based on 16S rDNA or orthologues AMETH_3452, novel actinobacterial class-specific conserved 62 18 type strains, respectively, three major phyletic lineages, namely mesophilic moderately thermophilic A. orientalis subclade (AOS), taiwanensis (ATS) (AMS). distinct growth temperatures members subclades correlated corresponding genetic variations in their compatible solutes. This study shows value integrating conventional taxonomic whole sequence data.