Bacterial floc mediated rapid streamer formation in creeping flows

作者: Mahtab Hassanpourfard , Zahra Nikakhtari , Ranajay Ghosh , Siddhartha Das , Thomas Thundat

DOI: 10.1038/SREP13070

关键词:

摘要: One of the central puzzles concerning interaction low Reynolds number fluid transport with bacterial biomass is formation filamentous structures called streamers. In this manuscript, we report our discovery a new kind Re streamers, which appear from pre-formed flocs. sharp contrast to biofilm-mediated these streamers form over extremely small timescales (less than second). Our experiments, carried out in microchannel micropillars rely on fluorescence microscopy techniques illustrate that floc-mediated when freely-moving floc adheres micropillar wall and gets rapidly sheared by background flow. We also show at their inception deformation flocs dominated recoverable large strains indicating significant elasticity. These subsequently increase tremendously produce Interestingly, find fully formed are not static viscous response time scales larger scales. Finally, such novel streamer can lead rapid clogging microfluidic devices.

参考文章(30)
Barry A. Friedman, Patrick R. Dugan, Robert M. Pfister, Charles C. Remsen, Structure of Exocellular Polymers and Their Relationship to Bacterial Flocculation Journal of Bacteriology. ,vol. 98, pp. 1328- 1334 ,(1969) , 10.1128/JB.98.3.1328-1334.1969
Jost Wingender, Thomas R Neu, Hans-Curt Flemming, None, What are Bacterial Extracellular Polymeric Substances Springer, Berlin, Heidelberg. pp. 1- 19 ,(1999) , 10.1007/978-3-642-60147-7_1
Hans-Curt Flemming, Thomas R. Neu, Daniel J. Wozniak, The EPS Matrix: The “House of Biofilm Cells” Journal of Bacteriology. ,vol. 189, pp. 7945- 7947 ,(2007) , 10.1128/JB.00858-07
Amin Valiei, Aloke Kumar, Partha P. Mukherjee, Yang Liu, Thomas Thundat, A web of streamers: biofilm formation in a porous microfluidic device Lab on a Chip. ,vol. 12, pp. 5133- 5137 ,(2012) , 10.1039/C2LC40815E
Roberto Rusconi, Sigolene Lecuyer, Nicolas Autrusson, Laura Guglielmini, Howard A. Stone, Secondary flow as a mechanism for the formation of biofilm streamers. Biophysical Journal. ,vol. 100, pp. 1392- 1399 ,(2011) , 10.1016/J.BPJ.2011.01.065
Aurélie Marty, Christine Roques, Christel Causserand, Patrice Bacchin, Formation of bacterial streamers during filtration in microfluidic systems. Biofouling. ,vol. 28, pp. 551- 562 ,(2012) , 10.1080/08927014.2012.695351
Minyoung Kevin Kim, Knut Drescher, On Shun Pak, Bonnie L Bassler, Howard A Stone, Filaments in curved streamlines: Rapid formation of Staphylococcus aureus biofilm streamers. New Journal of Physics. ,vol. 16, pp. 065024- 065024 ,(2014) , 10.1088/1367-2630/16/6/065024
Cynthia Wu, Ji Youn Lim, Gerald G. Fuller, Lynette Cegelski, Quantitative Analysis of Amyloid-Integrated Biofilms Formed by Uropathogenic Escherichia coli at the Air-Liquid Interface Biophysical Journal. ,vol. 103, pp. 464- 471 ,(2012) , 10.1016/J.BPJ.2012.06.049
P Stoodley, R Cargo, C J Rupp, S Wilson, I Klapper, Biofilm material properties as related to shear-induced deformation and detachment phenomena Journal of Industrial Microbiology & Biotechnology. ,vol. 29, pp. 361- 367 ,(2002) , 10.1038/SJ.JIM.7000282