Identification of abnormal fucosylated-glycans recognized by LTL in saliva of HBV-induced chronic hepatitis, cirrhosis, and hepatocellular carcinoma.
作者： Jiaxu Zhang , Yaogang Zhong , Peixin Zhang , Haoqi Du , Jian Shu
关键词:
摘要: The hepatitis B virus (HBV)-induced chronic liver diseases are serious health threats worldwide. There is evidence to display the alterations of salivary N-linked glycans related development HBV-infected diseases. Here, we further investigated fucosylated N/O-glycans recognized by LTL in saliva from 120 subjects (30 healthy volunteers (HV), 30 patients with (HB), hepatic cirrhosis (HC), and hepatocellular carcinoma (HCC)) using microarrys MALDI-TOF/TOF-MS. results showed that expression level was significantly increased HCC compared other (P < 0.0001). Besides, glycoproteins were isolated pooled HV, HB, HC, LTL-magnetic particle conjugates. Then, N/O- released PNGase F NaClO, identified MALDI-TOF-MS, respectively. Totally, there 21/20, 25/18, 29/19, 28/24 N/O-glycan peaks annotated proposed structures HCC. Among total, 8 N-glycan (e.g., m/z 1905.634, 2158.777 2905.036) 15 O-glycan 1177.407, 1308.444 1322.444) only presented HBV-induced One peak (m/z 2205.766) unique 9 1157.420, 1163.417 1193.402) This study could facilitate discovery biomarkers for HC based on precise saliva.
oup.com
sci-hub.se 参考文章(42)
T.P. Vavilova, I.G. Ostrovskaya, A.E. Medvedev, [Lecture: prospects of hormone analyses in saliva]. Biomeditsinskaya khimiya. ,vol. 60, pp. 295- 307 ,(2014) , 10.18097/PBMC20146003295
Yaogang Zhong, Yannan Qin, Hanjie Yu, Jingmin Yu, Haoxiang Wu, Lin Chen, Peixin Zhang, Xiurong Wang, Zhansheng Jia, Yonghong Guo, Hua Zhang, Junjie Shan, Yuxia Wang, Hailong Xie, Xiaojie Li, Zheng Li, Avian Influenza Virus Infection Risk in Humans with Chronic Diseases Scientific Reports. ,vol. 5, pp. 8971- 8971 ,(2015) , 10.1038/SREP08971
Garima Gupta, Avadhesha Surolia, Srinivasa-Gopalan Sampathkumar, Lectin Microarrays for Glycomic Analysis Omics A Journal of Integrative Biology. ,vol. 14, pp. 419- 436 ,(2010) , 10.1089/OMI.2009.0150
Anand Mehta, Timothy M. Block, Fucosylated glycoproteins as markers of liver disease Disease Markers. ,vol. 25, pp. 259- 265 ,(2008) , 10.1155/2008/264594
Howard J. Allen, Edward A. Z. Johnson, Khushi L. Matta, A Comparison of the Binding Specificities of Lectins from Ulex Europaeus and Lotus Tetragonolobus Immunological Communications. ,vol. 6, pp. 585- 602 ,(1977) , 10.3109/08820137709093469
Ye Wang, Shisheng Sun, Tianran Ma, Ting Wang, Qiaoling Chen, Zheng Li, Ganglong Yang, Ting Cui, Selective isolation and analysis of glycoprotein fractions and their glycomes from hepatocellular carcinoma sera Proteomics. ,vol. 13, pp. 1481- 1498 ,(2013) , 10.1002/PMIC.201200259
Mary Ann Comunale, Melissa Lowman, Ronald E Long, Jonathan Krakover, Ramila Philip, Steven Seeholzer, Alison A Evans, Hie-Won L Hann, Timothy M Block, Anand S Mehta, None, Proteomic Analysis of Serum Associated Fucosylated Glycoproteins in the Development of Primary Hepatocellular Carcinoma Journal of Proteome Research. ,vol. 5, pp. 308- 315 ,(2006) , 10.1021/PR050328X
RajkumarN Parwani, SimranR Parwani, Quantitative evaluation of serum fucose in oral squamous cell carcinoma patients. Journal of Cancer Research and Therapeutics. ,vol. 7, pp. 143- 147 ,(2011) , 10.4103/0973-1482.82928
Salomé S. Pinho, Sandra Carvalho, Ricardo Marcos-Pinto, Ana Magalhães, Carla Oliveira, Jianguo Gu, Mário Dinis-Ribeiro, Fátima Carneiro, Raquel Seruca, Celso A. Reis, Gastric cancer: adding glycosylation to the equation Trends in Molecular Medicine. ,vol. 19, pp. 664- 676 ,(2013) , 10.1016/J.MOLMED.2013.07.003
Joseph D. Foley, J. Darrell Sneed, Steven R. Steinhubl, Justin Kolasa, Jeffrey L. Ebersole, Yushun Lin, Richard J. Kryscio, John T. McDevitt, Charles L. Campbell, Craig S. Miller, Oral fluids that detect cardiovascular disease biomarkers Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. ,vol. 114, pp. 207- 214 ,(2012) , 10.1016/J.OOOO.2012.03.003