Comparative analysis of two 16S rRNA gene-based PCR primer sets provides insight into the diversity distribution patterns of anammox bacteria in different environments

摘要: Due to the high divergence among 16S rRNA genes of anammox bacteria, different diversity pattern community could be resulted from using primer set. In this study, efficiencies and specificities two commonly used sets, Amx368F/Amx820R Brod541F/Amx820R, were evaluated by exploring characteristics bacteria in sediments marine, estuary, freshwater wetland. Statistical analysis indicated that base mispairing rate between bases on gene sequences retrieved their corresponding ones Brod541F was quite high, suggesting efficiency specificity Brod541F/Amx820R. Further experimental results demonstrated multiple genera including Ca. Scalindua, Brocadia, Kuenenia, able detected Amx368F/Amx820R, but only Scalindua Moreover, phylogenetic clusters completely those presenting a significant complementary effect. By joint application these distribution patterns environments analyzed. Almost all marine belonged Scalindua. Sequences wetland affiliated Brocadia new clusters, while found river-sea intersection environmental feature. total, prime sets have characteristic for detecting samples, combined achieve better display community.