Gene analysis method using sdl-pcr

摘要: The present invention relates to a gene analysis method using SDL-PCR (separation of displaced ligation probe-based PCR), and, more specifically, SDL-PCR, wherein various probes comprising base sequences which are complementary target subjected reaction by ligase, and different probe able hybridize with the said is added hybridization after an extension carried out so as produce template probe, amplified common primer. In according invention, genomic DNA or unreacted have not been eliminated means tags, thus it possible minimise non-specific amplification reactions additionally, purification can be effected in shorter time than methods exonuclease, ligation, cleaning polymerase chain same solution single tube hence simultaneously amplify plurality genes fashion both accurate quick.