Computational and biological characterization of fusion proteins of two insecticidal proteins for control of insect pests.

摘要: Sucking pests pose a serious agricultural challenge, as available transgenic technologies such Bacillus thuringiensis crystal toxins (Bt) are not effective against them. One approach is to produce fusion protein for the control of these pests. Two toxins, Hvt (ω-atracotoxin from Hadronyche versuta) and onion leaf lectin, were translationally fused evaluate negative effects proteins on Phenacoccus solenopsis (mealybug), phloem-feeding insect pest. was cloned both N-terminally (HL) then C-terminally (LH) in constructs, which expressed transiently Nicotiana tabacum using Potato Virus X (PVX) vector. The HL found be more P. solenopsis, with an 83% mortality rate, compared LH protein, caused 65% mortality. lectin alone 42% 45%, respectively, under same conditions. Computational studies showed that stable than protein. Together, results demonstrate translational two insecticidal improved activity relative each individually could plants sucking