In vitro invasion capacity of Salmonella Typhimurium DT9 isolates sourced from humans and layer hen environments.

摘要: In Australia, Salmonella Typhimurium definitive type 9 is frequently isolated during foodborne outbreaks of salmonellosis. Multiple-locus variable number tandem repeat analysis (MLVA) trace back investigations identify isolate distribution patterns that may be epidemiologically linked to disease outbreaks. this study, the in vitro virulence potential S. DT9 isolates possessing different MLVA (03 15 07 11 550, 03 24 10 523, 08 550 and 14 550) from either humans or layer hens was assessed using a human colon carcinoma cell line. Four strains per each host for total 32 were included these experiments. Bacteria grown stationary phase added cells at multiplicity infection 100. Across all isolates, mean percent recovery ranged 7.1 ± 1.1 33.3 ± 7.1%. The hen isolate, KC900 (MLVA profile 550), exhibited greatest invasion with Overall, had significantly higher into Caco2 than (p = .0021). RAPD enterobacterial repetitive intergenic consensus genomic fingerprinting also performed. Irrespective source, SalmonellaDT9 study similar fingerprint patterns.