Proton nuclear magnetic resonance spectroscopy unambiguously identifies different neural cell types

摘要: Proton nuclear magnetic resonance (1H NMR) spectroscopy is a noninvasive technique that can provide information on wide range of metabolites. Marked abnormalities 1H NMR brain spectra have been reported in patients with neurological disorders, but their neurochemical implications may be difficult to appreciate because data are obtained from heterogeneous tissue regions composed several cell populations. The purpose this study was examine the profile major neural types. This helpful understanding metabolic detected by spectroscopy. Extracts cultured cerebellar granule neurons, cortical astrocytes, oligodendrocyte-type 2 astrocyte (O-2A) progenitor cells, oligodendrocytes, and meningeal cells were analyzed. purity > 95% all lineages, except for neurons (approximately 90%). Although constituents (creatine, choline-containing compounds, lactate, acetate, succinate, alanine, glutamate) ubiquitously detectable NMR, each type had distinctive qualitative and/or quantitative features. Our most unexpected finding large amount N-acetyl-aspartate (NAA) O-2A progenitors. compound, consistently vivo, previously thought ne present only neurons. an alanine:creatine ratio three four times higher than or oligodendrocytes agreement observations meningiomas express gliomas. suggest individual has characteristic pattern discriminated even looking at few metabolites (e.g., NAA, glycine, beta-hydroxybutyrate).(ABSTRACT TRUNCATED AT 250 WORDS)