Patterns of ENOD40 gene expression in stem-borne nodules of Sesbania rostrata

作者: Viviana Corich , Sofie Goormachtig , Sam Lievens , Marc Van Montagu , Marcelle Holsters

DOI: 10.1023/A:1005925607793

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摘要: At the base of adventitious root primordia, located on stem tropical legume Sesbania rostrata, nitrogen-fixing nodules are formed upon inoculation with microsymbiont Azorhizobium caulinodans. This pattern nodule development presents features indeterminate and determinate in early later stages, respectively. A S. rostrata cDNA clone homologous to nodulin ENOD40 genes was isolated from a library developing nodules. SrENOD40-1 contained conserved regions I II other genes. By reverse transcriptase PCR, enhanced expression observed primordia between 4 8 h after A. In situ hybridization showed that transcripts, present around central vascular bundle uninfected were strongly induction development. De novo initiating growing bundles. When cell type specification sets in, became pronounced cells derived meristematic regions. parts plant, weak associated bundles leaf stipule primordia.

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