Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells.
作者： H.E. Grecco , K.A. Lidke , R. Heintzmann , D.S. Lidke , C. Spagnuolo
DOI: 10.1002/JEMT.20129
关键词:
摘要: In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Forster resonance energy transfer (FRET); (5) spectral conversion of exposed to high-intensity illumination. also demonstrate the utility imaging binding uptake biotinylated transferrin on living cells, resolving lifetime microscopy (FLIM) signals originating from those spatially spectrally overlapping visible fluorescent proteins (VFPs).
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