Studies and critique of amido black 10B, Coomassie blue R, and fast green FCF as stains for proteins after polyacrylamide gel electrophoresis
作者： Curtis M. Wilson
DOI: 10.1016/0003-2697(79)90581-5
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摘要: The properties of amido black 10B (CI 20470), Coomassie blue R (CI 42660), and fast green FCF (CI 42053) as protein stains, along with a few comments on Coomassie blue G (CI 42655), are presented and dye impurities and their effects on protein-dye binding within gels are discussed. All three dyes produced metachromatic effects with some proteins. Problems encountered with long-term stability and fixation of certain maize seed proteins are reported along with procedures for overcoming them. The low solubility of Coomassie blue R in …
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