Transient gene expression in tobacco protoplasts: I. Time course of CAT appearance.
作者： Markus Pröls , Reinhard Töpfer , Jozef Schell , Hans-Henning Steinbiß
DOI: 10.1007/BF00272528
关键词:
摘要: The early events of transient gene expression have been investigated monitoring CAT activity in tobacco protoplasts encoded by the recombinant plasmid pRT101cat. first appearance was observed within 30 minutes after outset cultivation, and maximal values were obtained between four 24 hours. expression, at level RNA synthesis, could not be inhibited cordycepin (3'deoxyadenine) added one hour protoplast plating, whereas cycloheximide, an inhibitor protein showed influence during This indicates a rapid decay biologically active forms both DNA transferred CAT-mRNA synthesized These results suggest that system stability lasts up to two weeks rather than continuous synthesis new enzyme.
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