Analysis of the genomic organisation of a small chromosome of Leishmania braziliensis M2903 reveals two genes encoding GTP-binding proteins, one of which belongs to a new G-protein family and is an antigen

摘要: Leishmania braziliensis M2903 contains a highly amplified small chromosome. This work is aimed at resolving its structural organization and determining whether this unusual chromosome specific genes encoding proteins with important functions in disease pathology or drug resistance. Our results show that the 250-kb LD1 sequences has an inverted repeat structure. The two cDNAs (cDNA2 cDNA53) were mapped on cosmid contig, corresponding genomic fragments from sequenced. gene cDNA2 predicts putative GTP-binding protein homology to other only G-1 domain region; however, four conserved motifs can be recognized. Sequence similarity cDNA53 located least five chromosomes, copy pseudogene. An open reading frame downstream of pseudogene another belongs new G-protein family newly characterized sequence motif. A portion was studied previously L. aethiopica as recombinant antigen reacts human antibodies.