A complex array of positive and negative elements regulates the chicken alpha A-crystallin gene: involvement of Pax-6, USF, CREB and/or CREM, and AP-1 proteins.
作者： A Cvekl , C M Sax , E H Bresnick , J Piatigorsky
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摘要: The abundance of crystallins (> 80% the soluble protein) in ocular lens provides advantageous markers for selective gene expression during cellular differentiation. Here we show by functional and protein-DNA binding experiments that chicken alpha A-crystallin is regulated at least five control elements located sites A (-148 to -139), B (-138 -132), C (-128 -101), D (-102 -93), E (-56 -41). Factors interacting with these were characterized immunologically gel mobility shift experiments. results are interpreted following model. Site binds USF part a composite element site B. CREB and/or CREM enhance an AP-1 complex including CREB, Fra2 JunD which interacts on repress fibroblasts. Sites (which conserved across species) bind Pax-6 stimulate promoter activity. These provide first direct data contributes lens-specific crystallin gene. (-104 -93) negative element. Thus, indicate USF, (or factors), array positive cis-acting high repression
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