Peptide-specific CD8+ T-cell evolution in vivo: response to peptide vaccination with Melan-A/MART-1.
作者： Elke Jäger , Hauni Höhn , Artje Necker , Reinhold Förster , Julia Karbach
DOI: 10.1002/IJC.10165
关键词:
摘要: Monitoring of CD8+ T-cell responses in cancer patients during peptide vaccination is essential to provide useful surrogate markers and demonstrate vaccine efficacy. We have longitudinally followed 3 melanoma who were immunized with peptides derived from Melan-A/MART-1. Recombinant HLA-A2 tetramers loaded the naturally presented Melan-A/MART-1 nonamer (AAGIGILTV) analog (ELAGIGILTV) used combination phenotypical analysis for different subsets including naive T cells, effector "true memory" cells "memory effector" based on CD45RA/RO CCR7-expression. At least a single patient, binding AAGIGILTV epitope detected naive, precursor (CD45RA+/CCR7+) ELAGIGILTV identified terminally differentiated (CD45RA+/CCR7-) subset. Molecular functional tetramer-binding revealed that receptor (TCR) repertoire was oligo/polyclonal AAGIGILTV-reactive but restricted few TCR clonotypes ELAGIGILTV-reactive prior vaccination. The reactive epitopes broadened exhibited profile cytokine release after specific stimulation: 2/3 secreted granulocyte/macrophage colony-stimulating factor (GM-CSF) interferon-gamma not interleukin-2 (IL-2) response peptides. In third IL-2 exclusively. Our results show consist associated functions. Complementary CDR3 profiles may be define most effective population induced by
elsevier.com
sci-hub.se 参考文章(53)
Marie Bodinier, Marie-Alix Peyrat, Claire Tournay, François Davodeau, François Romagne, Marc Bonneville, François Lang, Efficient detection and immunomagnetic sorting of specific T cells using multimers of MHC class I and peptide with reduced CD8 binding. Nature Medicine. ,vol. 6, pp. 707- 710 ,(2000) , 10.1038/76292
Peter P. Lee, Cassian Yee, Peter A. Savage, Lawrence Fong, Dirk Brockstedt, Jeffrey S. Weber, Denise Johnson, Susan Swetter, John Thompson, Philip D. Greenberg, Mario Roederer, Mark M. Davis, Characterization of circulating T cells specific for tumor-associated antigens in melanoma patients Nature Medicine. ,vol. 5, pp. 677- 685 ,(1999) , 10.1038/9525
Andrea Anichini, Giorgio Parmiani, Cristina Maccalli, Marialuisa Sensi, Cinthia Farina, TCR beta-chain variable region-driven selection and massive expansion of HLA-class I-restricted antitumor CTL lines from HLA-A*0201+ melanoma patients. Journal of Immunology. ,vol. 158, pp. 5902- 5913 ,(1997)
E. G. Engleman, C. J. Benike, L. M. Kawamura, N. Mohagheghpour, D. Gammon, A. Van Vollenhoven, CTL Response to Mycobacterium tuberculosis: Identification of an Immunogenic Epitope in the 19-kDa Lipoprotein Journal of Immunology. ,vol. 161, pp. 2400- 2406 ,(1998)
Daniel C. Douek, Richard D. McFarland, Philip H. Keiser, Earl A. Gage, Janice M. Massey, Barton F. Haynes, Michael A. Polis, Ashley T. Haase, Mark B. Feinberg, John L. Sullivan#, Beth D. Jamieson, Jerome A. Zack, Louis J. Picker, Richard A. Koup, Changes in thymic function with age and during the treatment of HIV infection Nature. ,vol. 396, pp. 690- 695 ,(1998) , 10.1038/25374
Philip D. Greenberg, Mark M. Davis, Peter P. Lee, Cassian Yee, Peter A. Savage, Isolation of High Avidity Melanoma-Reactive CTL from Heterogeneous Populations Using Peptide-MHC Tetramers Journal of Immunology. ,vol. 162, pp. 2227- 2234 ,(1999)
R. Lee Reinhardt, Alexander Khoruts, Rebecca Merica, Traci Zell, Marc K. Jenkins, Visualizing the generation of memory CD4 T cells in the whole body Nature. ,vol. 410, pp. 101- 105 ,(2001) , 10.1038/35065111
Paola Squarcina, Ettore Appella, Giorgio Parmiani, Licia Rivoltini, Niels Ødum, Chiara Castelli, Carsten Geisler, Douglas J. Loftus, Mai Britt Nielsen, Peptides Derived from Self-Proteins as Partial Agonists and Antagonists of Human CD8+ T-Cell Clones Reactive to Melanoma/Melanocyte Epitope MART127–35 Cancer Research. ,vol. 58, pp. 2433- 2439 ,(1998)
I Puisieux, P Kourilsky, J Even, F Jotereau, C Pannetier, M Favrot, Oligoclonality of tumor-infiltrating lymphocytes from human melanomas. Journal of Immunology. ,vol. 153, pp. 2807- 2818 ,(1994)
Giuseppe Pantaleo, Patrick Champagne, Graham S. Ogg, Abigail S. King, Christian Knabenhans, Kim Ellefsen, Massimo Nobile, Victor Appay, G. Paolo Rizzardi, Sylvain Fleury, Martin Lipp, Reinhold Förster, Sarah Rowland-Jones, Rafick-P. Sékaly, Andrew J. McMichael, Skewed maturation of memory HIV-specific CD8 T lymphocytes Nature. ,vol. 410, pp. 106- 111 ,(2001) , 10.1038/35065118